Recombinant Proteins
Backed by over 60 years of cumulative experience in the generation of recombinant proteins from E. coli and Mammalian cell cultures, we use advanced equipment to meet and exceed customer expectations. Over the years, our capabilities have grown to include generating research-grade and GLP-grade materials and organizing GMP material production to meet both clinical and commercial needs.
MOLECULAR BIOLOGY
Bacterial Expression:
Scouting for expression
(4-6 weeks)
Mammalian Expression:
Transient expression:
(4-6 weeks)
Stable expression
(20-30 weeks)
Gene Expression Analysis:
Q-PCR based
expression profiling
UPSTREAM
Bacterial Process Development:
Shake flask
Fermenter-5, 10 & 20L
(6-8 weeks)
Mammalian Process Development:
Shake flask
Bioreactor 1, 5, 10 & 1000L
(8-15 weeks)
DOWNSTREAM
Optimization:
Solubilization and refolding
(8 weeks)
Process Optimization:
Resin scouting & optimized process
(8 weeks)
Optimization:
Process optimization
Resin scouting
(6-8 weeks)
ANALYTICAL DEVELOPMENT
Complete analytical method
development and validation
Stability studies for DS under conditions (2-8oC, 25oC/60% RH 40oC/75% RH, – 80oC & -20oC)
High End Characterization
(product & process related impurities)
Bioassay development